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Dark Heart Nursery Discover Cannabis Virus HpLVd

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HpLVd

Growers around Californa are facing a virus problem, and one of California’s oldest cannabis nurseries, Dark Heart Nursery (DHN), has the antidote. With the help of Dr. Jeremy Warren, DHN discovered the HpLVd virus that has been plaguing growers for the past several years. Now, it’s time to clean up.

Cause of Cannabis “Dudding” Identified: HpLVd Virus

Throughout history, the cannabis plant has faced its fair share of viral infections. Repeated infections gave rise to genetic changes over time, altering the evolution of the plant. In fact, there is evidence that ancient viruses are at least in part responsible for the herb’s ability to produce THC.

For the past several years, however, viruses have taken a more sinister role in the plant’s life. Strains that once grew vigorously have started to show signs of ware — a drab yellow color replacing what should be a vibrant green. Misshapen leaves develop without warning, under conditions that would otherwise result in a thriving harvest. Growers developed their own term to describe the phenomenon, “dudding”.

These unusual symptoms were recorded in 2015, when Dr. Rick Crum found that as much as 35 percent of nursery samples demonstrated these symptoms, along with stunted growth. At the time, the cause of these symptoms was identified as “punitive cannabis infective agent” (PCIA), a term used to describe the unexplainable loss of vitality among cannabis plants.

Now, however, a team at DHN has finally identified a cause: the hop latent viroid. Also known as the HpLVd virus, DHN is the first organization to identify the precise cause of dudding.

From Clone to Clone

As reported by Cannabis Now, a group of underground researchers in California called Humboldt DNA articulated that PCIA appears to be passed along via pollen exposure and cloning of infected plants. This allows the virus to be transmitted quickly, infecting larger numbers of plants each year.

As you might guess from the name, hop latent viroid is a virus that traditionally attacks hop plants. Hops, which are used to make beer, are among the closest relatives to the cannabis plant. Throughout the mid-2000s, reports suggest that the HpLVd virus is spreading. In 2007, hop farmers in China filed the first statements suggesting that the virus was harming local harvests. The same virus causes similar symptoms in hops, and cannabis appears to be a prime new target.

The rapid spread of the virus was confirmed researchers at Dark Heart Nursery, who found that healthy plants exposed to infected clones were quick to “dud” themselves. The team that made the discovery was led by Jeremy Warren, Ph.D., who now works as DHN’s Director of Plant Health.

Clean Up Time

This same virus is now attacking cannabis plants, potentially causing major losses for medical and recreational cannabis growers alike. While the virus has been identified in cannabis, clear plans for eradication still illude many growers. Should the virus infect a large portion of a harvest, growers may be forced to dispose of their crops and decontaminate their soils and growing mediums.

Among identifying HpLVd, Dark Horse Nursery has developed a functioning “clean process” under the guidance of Research Manager Will Roberts. This process, however, is currently patent-pending, giving DHN ownership over the eradication processes. Apart from mimicking eradication processes used by the hop industry, however, many home growers and professional cultivators alike may still be left in the dark regarding how to properly eradicate HpLVd illness in their plants.

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Borneol: The Terpene That Improves Your Heart and Gut Health

This terpene Borneol offers many health benefits, including improved digestion (via the stimulation of gastric juices) and better blood circulation.

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Borneol
PHOTO | Jesse Barney

Of the 20,000 aromatic molecules called terpenes found throughout nature, roughly 200 have been discovered in the cannabis herb. As an aggregate, the most common health efficacies offered by cannabis-derived terpenes are reduced systemic inflammation, anti-cancer properties, and analgesic (pain-killing) benefits. The terpene Borneol emits an odor involving scents of camphor, earth, and menthol; its scent is sometimes described as “herbal minty.” It is found in many plants other than cannabis, including camphor, mint, and rosemary. It is a natural insect repellent and is utilized industrially in the manufacture of perfumes and colognes.

Borneol is not extremely common in cannabis. Strains of cannabis offering more significant amounts of this terpene include Hazes and K-13.

The Details of Borneol

This terpene offers many health benefits, including improved digestion (via the stimulation of gastric juices) and better blood circulation. It also effectively treats bronchial symptoms to improve lung function and ease breathing (helpful for sufferers of bronchitis and asthma). Like many of its sibling terpenes, it has been found to reduce anxiety.

Borneol also assists in the healing of wounds. Historically, it has been incorporated into topical treatments for such applications, including the treatment of hemorrhoids. Combined with other terpenes that convey sedative qualities — such as myrcene and linalool — the terpene can effectively combat insomnia. It is also antibacterial and antiseptic.

Borneol exemplifies the dynamics of the entourage effect, a theory that cannabinoids and terpenes work together, synergistically, to offer particular therapeutic and medicinal benefits to humans via supplementation of the mammalian endocannabinoid system (ECS). Borneol amplifies the permeability of the blood/brain barrier, allowing other molecules and compounds to more efficiently bind with specialized receptors in the brain and central nervous system.

When isolated, it can act as an irritant to eyes, skin, and the respiratory system. As a good demonstration of the impact of accurate and strategic titration (dosing), at large doses, it is extremely toxic and harmful if swallowed. A “probable lethal dose” is 50-500 mg/kg of body weight, which equates to between only a teaspoon and an ounce for a 150-pound human.

The Research

Multiple studies have demonstrated the wide-ranging medicinal efficacy of the terpene, including its pronounced role as an anti-inflammatory, bronchodilator, and cancer killer.

A 2017 study entitled “Terpenes from Forests and Human Health” and published in the journal Toxicological Research investigated how borneol reduced inflammation of the lungs. Concluded the researchers, “Borneol alleviated acute lung inflammation by reducing inflammatory infiltration, histopathological changes, and cytokine production.”

A 2013 study published in the journal PLOS ONE and entitled “Natural Borneol, a Monoterpenoid Compound, Potentiates Selenocystine-induced Apoptosis in Human Hepatocellular Carcinoma Cells” revealed the anti-cancer properties of this terpene.

The study’s researchers concluded, “Borneol effectively synergized with SeC to reduce cancer cell growth through the triggering apoptotic cell death. These results reveal that borneol strongly potentiates SeC-induced apoptosis in cancer cells by enhancement of cellular uptake. Borneol could be further developed as a chemosensitizer of SeC in treatment of human cancers.”

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There Needs to Be More Research on How Cannabis Affects Dreams

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How Cannabis Affects Dreams
PHOTO | Jesse Barney

Most people forget their dreams shortly after waking. As soon as the eyelids flutter open, an entire evening of vivid adventures and abstract situations shuffle to an end, often leaving only lingering traces of their presence — a fragment of a dress here, the flashing face of a stranger there. While some individuals may be adept at remembering these mysterious nocturnal encounters, for the most part, the dreaming mind remains an enigma.

For those who consume cannabis, the dreaming self may remain even more elusive. The plant, you see, may prevent the sleeping mind from dreaming. Although cannabis has long been used as a meditation aid and sleep-inducer, preliminary research suggests the trance-inducing herb may suppress some forms of sleep consciousness. Of course, however, research on how cannabis affects dreams is far too premature to make any serious assessments.

The Purpose of Dreaming

Dream science over the past several decades provides more and more hints into the true nature of the dreaming mind. While the exact purpose of dreaming is unclear and dreams are particularly difficult to study, it is generally understood that the dreaming mind is also an emotional mind. While logic and rationality dominate during the day, the unique function of the dreaming mind is to help soothe and come to terms with emotional memories.

Most dreaming occurs during rapid eye movement sleep (REM sleep). The REM cycle is the closest sleep state to wakefulness when electrical activity in the brain increases, along with heart rate and breathing. During this time, levels of bodily stress hormones drop to their lowest levels. During the day, the hormone norepinephrine increased in the bloodstream in response to everyday stressors and anxieties. At night, however, norepinephrine levels slowly decrease as the dreaming mind takes over.

Brain scans suggest that the dreaming mind is very similar to the waking mind; visual areas of the brain are highly engaged, as well as areas related to memory and conscious thinking. A primary difference, however, is that the waking mind also responds to stress signals by releasing hormones like norepinephrine for fight-or-flight response. When dreaming, even strong emotions are distanced from this fight-or-flight impulse of the nervous system.

While the content of dreams may be very different than your visual memories from everyday life, it appears that emotional memory processing is a core function of REM sleep. The situations that your dreaming self is exposed to may be abstract or downright weird, but the emotional context of the dream may be what holds real value.

How Cannabis Affects Dreams

If dreams are the brain’s way of de-escalating and filing away emotional memories, then the effects of cannabis on dreams are well worth knowing. Thus far, a multitude of preclinical and small human trials show that cannabis consumption causes a reduction in REM sleep. This reduction is most strongly correlated with THC, although animal research thus far hasn’t determined whether or not the same effect occurs with CBD.

For individuals with recurring nightmares, this could be a positive benefit. Those with post-traumatic stress disorder (PTSD), for example, often demonstrate disturbed REM sleep patterns and may consistently remember their dreams. Healthy sleep is sleep that promotes feelings of restfulness upon waking. If a person plagued by chronic nightmares, it can have a distressing impact on the next wakeful day

In PTSD, patients are unable to delete fear-based experiences from their memories. Already, preclinical research suggests that cannabis medicines may be beneficial for those with the condition, potentially helping patients extinguish fear-based memories during the waking day.

And yet, for the everyday individual, the overall effect of cannabis on dreams remains unknown. Research suggests that the plant does, in fact, reduce dreams and limits the amount of time spent in REM sleep. Given that REM sleep is when the body records and desensitizes emotional memories, it’s possible that reducing time spent in REM may have a negative effect on emotional processing.

The catch? Very little research has been conducted on the aftermath of cannabis consumption on dreaming. Although cannabis is one of the most popular illicit sleep aids in the world, no high-quality or conclusive evidence exists on the impact of cannabis on sleep. Further, the bulk of research on the topic was conducted in the 1970s and 1980s, with isolated cannabis compounds and small participant numbers. As a result, the way in which cannabis affects dreaming and the implications this may have on waking life are simply unknown.

REM Rebound

The human brain does not like to go without enough REM sleep. While quality research on cannabis and dreaming is appallingly absent from the scientific literature, what is known, however, is that dreams come back with a vengeance after a brief period of abstinence from the plant. When regular cannabis consumers abstain from the herb, one of the most common side effects of withdrawal is the resurgence of very vivid and memorable dreams. This phenomenon has a proper name: REM rebound.

During REM rebound, a person is able to reach a state of REM sleep more quickly. They’re also more likely to remain in REM sleep for an extended amount of time. This dreaming rebound most often occurs after a person experiences sleep deprivation. The brain, it would seem, opens the floodgates of emotion once you’ve finally safe to hit the hay.

In terms of REM sleep, does cannabis produce similar effects to sleep deprivation? Without more research on how cannabis affects dreams, it’s hard to say.

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Wizard Stones: The Magic of Making Cannabis Diamonds

Aaron Palmer and Graham Jennings, founders of Oleum Extracts in Washington State, talk about Wizard Stones, their THCA isolate product.

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Wizard Stones
PHOTO | Oleum Extracts

Heat, pressure, and time. The three components required to form a diamond from carbon. But what about diamonds made from cannabis? The founders of Oleum Extracts, Aaron Palmer and Graham Jennings both agree that a good diamond product ultimately comes down to the flavor provided by its terpene fraction.

Diamonds is a slang term for the crystal formations of the cannabinoid THCA. The molecule’s lattice structure builds upon itself naturally as individual molecules clump together creating the faceted formations that resemble diamond or quartz.

When most people talk about cannabis diamonds, they’re talking about THCA structures that form in their own terpene sauce. So, it’s a little different technique than other isolation methods.

Oleum —While their chemical composition is the same, the process to make them is slightly different than the traditional diamonds mined from a raw extract. Instead, they use a specially formulated solvent mix to create a solution with a composition that encourages crystallization.

Due to Washington state’s regulations, Oleum is limited in the chemical solvents they can use. So that blend is the crucial variable to isolating THCA into their Wizard Stones product.

Growing cannabis diamonds within their original terpene fraction comes down to creating an environment with the right amounts of pressure and heat to encourage crystal growth.

Within the raw extract, the terpene and cannabinoid compounds are homogenized together, but as they settle and separate the mixture “crashes” — which is the start of crystallization.

Palmer explains that it “helps to create a seed because if there’s nothing for the THCA molecules to grab onto then they have a harder time starting the diamond formation.”

There are a few ways extractors seed a solution to start diamond growth, but Oleum prefers to use freezing temperatures to solidify and then thaw their extract, helping to create small groupings of THCA for other molecules to stack off.

Another common seeding technique is to drop a previously grown crystal into the extracted mixture of cannabis compounds, giving the THCA something to grow off of.

This technique is especially useful when filming a time-lapse of the crystal growth because it gives the camera a focal point knowing where the formation will grow from.

Creating Wizard Stones

The above timelapse video wasphotographed over a four day period by Dankshire. We can see diamonds begin to form almost immediately. However, the crystallization process can take a month if not longer to complete once a raw extract is jarred and waiting to crash.

Oleum utilizes custom-built isolation vessels for their production diamond runs but admits that the jar tech allows more visibility into the process.

Jennings points out, “You see the jars, we even do the jar stuff a lot. It’s more popular… and people know what it is compared to a large isolation vessel that no one can see into it but you know it’s growing 2,000 grams of crystals.”

Each batch can present a different ratio of diamonds to sauce and it seems like everyone wants a little different combination. “We just give ‘em what it makes,” Jennings said.

That’s the beauty of isolated products like cannabis diamonds and sauce; you can mix your own cocktail of cannabis compounds and really dial in the flavors and feelings that you’re after.

Cannabis diamonds grown in their own sauce create a potent, refined, and pronounce expression of the strain they are extracted from.

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