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Terpinolene: The Powerful Medicinal Terpene with Anti-Cancer Promise

Terpinolene (sometimes denoted as “TPO”) is considered a minor, or secondary, terpene and features an aroma resembling sweet pine.

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Terpinolene
PHOTO | Pexels

Few cannabis consumers are fully aware of the complex molecular chemistry of the herb they vaporize, smoke, or eat. Terpenes like terpinolene are the aromatic molecules responsible for the sometimes revealingly pungent aroma of many strains of cannabis. Although the chief purpose of this family of chemicals is an evolutionary defense mechanism against pests and predators, terpenes have been found to deliver medicinal efficacy that equals that of cannabinoids such as CBD and THC.

More than 20,000 terpenes exist throughout nature, about 200 of which have been identified in various strains of cannabis. Terpinolene (sometimes denoted as “TPO”) is considered a minor, or secondary, terpene and features an aroma resembling sweet pine.  It delivers a taste reminiscent of citrus and is produced in many plants in nature other than cannabis, including allspice, cumin, junipers, parsnip, rosemary, sage, and tea tree.

The Details of Terpinolene

In terms of medicinal efficacy, terpinolene kills cancer cells and acts as a sedative. In addition, it displays antibacterial properties and is both an anti-fungal and an anti-oxidant. It is employed as a flavoring agent by various segments of the food industry. It is also used to manufacture plastics and resins and is an effective repellent against pests such as mosquitoes and weevils.

A 2013 study found this special molecule to be a powerful anti-cancer agent. Concluded the study’s researchers, “Our findings clearly demonstrate that terpinolene is a potent antiproliferative agent for brain tumour cells and may have potential as an anticancer agent, which needs to be further studied.”

The Research

A 2013 study entitled “Anticancer and Antioxidant Properties of Terpinolene” that was published in the journal Arh Hig Rada Toksikol found terpinolene to be a “potent antiproliferative agent for brain tumour cells” and surmised that this powerfully medicinal terpene “may have potential as an anticancer agent,” an issue in obvious need of further research due to the 91 million people worldwide who suffer more than 100 types of cancers.

The researchers concluded, “Our findings clearly demonstrate that terpinolene is a potent antiproliferative agent for brain tumor cells and may have potential as an anticancer agent, which needs to be further studied.”

A 2012 study entitled “Terpinolene, a Component of Herbal Sage, Downregulates AKT1 Expression in K562 Cells” and published in the journal Oncology Letters found the terpene terpinolene to be an effective agent in the battle against a variety of cancers.

Concluded the study’s researchers, “Increased expression and/or activation of AKT is involved in a variety of human cancers. In cells treated with sage or rosemary extract, mRNA and protein expression levels of AKT1 were reduced compared with those of the control cells 48 hours after the herbal treatments. We found that terpinolene, a common component of sage and rosemary, markedly reduced the protein expression of AKT1 in K562 cells and inhibited cell proliferation.”

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Cannabis

Here’s What You Need to Know If You’re New to Dabbing

Dabbing is an ideal ingestion method best for those that have a high tolerance to cannabis or patients that need a quick, controlled dose of cannabis.

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Dabbing
PHOTO roxxyphotos

Dabbing is an ideal ingestion method best for those that have a high tolerance to cannabis or for medical users that need a quick, concentrated and controlled dose of cannabis. However, it can be confusing, even for long-time pot smokers. In fact, unless you’ve done it, seen it, or read up on it, dabbing can be a total mystery.

Dabbing is a relatively new way to consume cannabis and it is has become very popular in recent years despite rumors that it is dangerous.  It is a highly concentrated experience, with THC at levels much more elevated than most regular flower you would encounter in a joint.  For patients, dabbing can be a very effective way to dose because the effects hit the user very quickly and can typically be measured more easily. For those with a high tolerance for cannabis, dabbing can be a way of feeling the effects of pot with more potency.

Before you can get into dabbing, you need to know a little about cannabis concentrates and extracts. Shatter, budder, wax, crumble, pull and snap, and hash oil are a few of the most popular types of cannabis concentrates and extracts. Extracts and concentrates are named so because they are products of a process where THC and other cannabinoids are extracted from the flower. Sometimes, during the extraction process, a solvent (like alcohol or butane) can be used and other times a solvent is not used. Either way, the final product is a smaller, stickier package that packs a powerful punch.

The Dabbing Process

Keep in mind that nails and domes can get incredibly hot. Like, RED hot, literally. Do not underestimate the heat that can occur — be cautious to prevent any burns.

First, you will need something to dab. We have heard the recommendation more than once to keep away from alcohol-based extracts when dabbing. Consult your budtender about this one, or just skip alcohol based-extracts — your call. There are a lot of concentrates and extracts to choose from, enjoy the hunt for your perfect pick.

Next you need something to dab out of. You can purchase a dab rig or just convert an existing glass water pipe with glass dab attachments. You are also going to need a titanium, ceramic, quartz or electronic nail that fits the glass dab attachment you are using. A typical nail is going to require the use of a dome in order to trap the vapor before it dissipates into the air. A dome can be as simple as a glass piece that fits over the reservoir where the extract or concentrate is vaporizing.

Get your dabber ready. A dabber is a tool that is ceramic, metal, glass, that is used to place the dab, or concentrate/extract, on the super-hot nail.

Lastly, unless you are using an electronic nail or e-mail, you will need a mini torch. Some less patient dabbers will use a full-on, propane-fueled torch in order to more quickly heat their nails — experiment at your own risk. In case the dab is a little overwhelming for you and your body, the safest place for you to be is sitting down to avoid any falls.

Turn on your e-nail OR use your torch to heat your nail until it is red-hot. Allow it cool for at least 10 seconds (for titanium) and up to 45 seconds if you are using ceramic or quartz nail.

Once cooled, use your dabber to place your concentrated dab on the nail. Place the dome over the nail as you inhale. Then, exhale. Victory!

If you weren’t already sitting down, you probably will be now!

Take these words of advice to heart — start small. If you haven’t tried dabbing at all before, don’t make your very first dab a large one. You won’t regret taking a small dab, but you might regret taking one that is too big. Always ask your budtender any questions you have about your purchase and if the product you are buying is the best thing you can buy for dabbing, vaporizing, smoking, etc.

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Plant Growth Regulators: What They Are & Why You Should Avoid Them

Plant Growth Regulators boost cannabis yields by pumping the nugs full of water at the cellular level which leads to the expansion.

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Plant Growth Regulators
PHOTO | lovelyday12

The dark years of cannabis prohibition in California created an innovative and highly competitive black market for weed that thrives to this day despite the state’s relatively progressive path toward legalization. Risk has always been high for illicit growers and their tactics to avoid the prying eyes of law enforcement have evolved over time. Decades ago, the use of helicopters and spotter planes to target pot farms forced growers to camouflage their cultivation, or in many cases, move it all the way indoors.

This radical change in the way cannabis was grown, particularly in Cali, led to a massive leap in quality control as indoor growers could now “play god” and manipulate all environmental conditions to their liking. However, it also led to a drastic drop in overall yields.

Remember, we are talking about the Nineties, so most of these were not yet warehouse-style grows. They were closets, garages, basements, and spare rooms for the most part and even though you could flip each room a few times per year, the overall weight harvested paled in comparison to the massive trees that could be grown outdoors.

With a limited footprint to grow in, and a limited canopy above (ie. the ceiling and lights), growers began looking for any way they could to boost the number of grams of finished buds that they could pull from each square foot of cultivation space.

For some, like our friend Josh D, that meant revolutionizing hydroponic grow systems and optimizing that method for cannabis production while at the same time introducing and perfecting the ultimate indoor strain — OG Kush. The optimal genetics of this iconic strain naturally produced shorter, bushier plants with massive, dense, and potent buds, making it ideal for indoor cultivation.

Not all growers were blessed with such genetics or know-how, though, and it didn’t take long for some of them to start to seek out some rather unsavory store-bought shortcuts to try to compensate for lackluster harvests.

Plant Growth Regulators (PGRs)

Plant Growth Regulators (PGRs) date back well before the Nineties cannabis boom. First introduced to American agriculture in the 1920s, these chemical compounds were used for nearly half a century to either boost or slow down the natural rate of growth of plants through phytohormonal manipulation.

For example, groves of fruit or nut trees could be filled with shorter trees that yielded more produce thereby reducing the time and risk of harvesting and, in turn, increasing profits.

However, when independent lab studies showed that certain synthetic PGRs could potentially be carcinogenic, the FDA stepped in and banned the use of such products on consumable food crops in the 1970s.

Those products, however, did not vanish. They continued to be used to keep trees at parks from growing too tall, or to make bouquets of grocery store flowers bloom brighter and grow at such uniform sizes and, as we know, to artificially boost cannabis yields. In fact, the global demand for PGRs has more than doubled in the past six years and now stands as a $6.4 billion sector of the agricultural industry.

Some growers are using this particular shortcut knowingly but many others may be pumping their pot full of PGRs without even knowing it as this controversial compound has been found (often unlabeled) in many popular brands of fertilizers and nutrients. If growers use those products and just so happen to see tighter, denser, and heavier nugs as a result… win/win, right? What’s wrong with increased yields and solid buds, anyway?

Guaranteed Mids

It’s no secret that, for the most part, the regulated cannabis market in California is flooded with mid-grade weed that is barely worthy of a blunt wrap for most seasoned smokers. The logistics of the supply chain and the way things have to be packaged in today’s market can mutilate even the best-grown buds by the time they reach the end-user but, sadly, most of the supply these days was pretty shoddy to begin with. We call it California’s “Mids Life Crisis”.

As many state-sanctioned cultivators struggle to stay afloat in the new legal market, too many are feeling the pressure of whatever deal they signed with the devil to fund their startup and are willing to do whatever it takes to make their numbers make sense. PGRs are the perfect tool for such an unscrupulous job.

Simply put, the way that PGRs boost cannabis yields is by pumping those nugs full of water at the cellular level which leads to the expansion. You are not boosting the vigor or potency of the plant, that added weight is literally moisture and cellulose. So while California cannabis testing labs are not required to flag samples for PGR levels, they will show lower overall THC levels — bigger buds do not always equal stronger buds.

So how can you tell if that herb you are inspecting at the dispensary has been treated with Plant Growth Regulators? Here are a few telltale signs to look for:

  • Buds that are extremely hard or dense in structure
  • Buds with an excessive amount of pistils, often matted or intertwined
  • Pistils often have more of a brown hue than the rusty orange seen on healthy buds
  • Distinct lack of visible developed trichomes
  • Lack of strain-appropriate aroma

Still unsure? Ask the budtender direct questions about it. Who grew it? Where was it grown? Research that feedback or pass on those products if that information is unknown. At the end of the day, it is our job as consumers to do the due diligence to ensure that what we put into our bodies is legit. Relying on any company or corporation to do that for you is naïve, particularly when it comes to the Wild West of legal weed.

Safety Meeting

Roughly a decade after the FDA banned the use of synthetic Plant Growth Regulators on crops meant for human consumption, the Environmental Protection Agency followed suit in the 1980s by placing even heavier restrictions on the use of certain PGRs and labeling them as environmental pollutants. The EPA warned that exposure to these synth PGRs could elevate a person’s risk of cancer 240 times higher than the acceptable standard.

That being said, many of those studies involved subjecting rats to astronomical levels of various PGRs in order to trigger negative reactions and there is no hard evidence that smoking weed treated with PGRs is much different than eating In n’ Out instead of a home-cooked burger. But, to us, it does matter and we will avoid synthetic PGR weed at all costs. We don’t want to smoke it, we don’t want to vape it, and we certainly have no interest in extracting it to create full-spectrum cannabis oil. Nothing about Plant Growth Regulators benefits the consumer, period.

Now, that’s not to say that all PGRs are bad news. In fact, there are many natural sources for Plant Growth Regulators that can be useful and healthy supplements for your fertilizer or nutrient base. But as a grower, you should feel obligated to thoroughly understand how they are sourced from nature and exactly how they work with the balanced chemistry of your plants before putting them to use. Failure to do so can take you from “Top Shelf” to “Midzotics” real quick!

Remember, fellow cultivators: If your SOP calls for more PGRs, then your QC is probably BS.

What’s Next?

Look for PGRs to make a major splash in the newly established American hemp marketplace as the importance of higher yields compounds dramatically at the large scale that those farms will be operating at. Combine that with the relative lack of lab testing requirements and Plant Growth Regulators figure to play a large role in helping the U.S. compete in the global market. American hemp is mandated by federal law to produce a ridiculously low 0.3% THC content, so the fact that PGRs murder trichomes only helps growers and manufacturers.

As for what role Plant Growth Regulators will play in the future of cannabis, that will likely come down to regulation since we see that there are too many growers who are willing to cut that corner when it is left up to them. At the very least, plant fertilizer and nutrient companies should be mandated by law to accurately list all ingredients and contents of their products. That way the growers who are trying to do the right thing can make informed decisions about how they treat their gardens, farms, and warehouse grows.

Finally, as consumers, we can speak with our hard-earned dollars. Quit buying PGR tainted buds! Tell your favorite dispensary that you aren’t interested in buying those products. If enough of us do exactly that, the free market will… ahem… weed out the PGR growers and those who push their products.

Beard Bros. Pharms has earned their reputation as a trusted source for cannabis news, content creation, and culture preservation. With decades of experience in cultivation and marketing, their fearless voice for the plant includes advocacy for veterans, inmates, people of color, and anyone else who has been oppressed by generations of cannabis prohibition. See what they’re up to now at BeardBrosPharms.com

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The Mighty Trichome: From Bag Appeal to a Billion Dollar Business

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Trichome
PHOTO | Antonio Romero

There are an ever-increasing amount of methods to ingest cannabis these days, but it all comes down to the mighty trichome and the microverse of molecules swirling inside. Our knowledge of the importance of the trichome is still developing, but let’s take a trip back to the good ol’ days… back when eyeballing an eighth was an earned skill and legendary strains like Hawg’s Breath, Bullrider, and White Widow were pushed in ziplock bags out of the backpacks and trunks of your local neighborhood hustler.

If you’ve ever scored weed on the streets you can probably relate, and in those wilder days money and product often changed hands quickly, leaving the buyer with little time for any sort of inspection of the goods. We were trained to hone in on certain aspects that collectively took on a name of their own: “bag appeal”.

Our crew has always sought out the best of the best, and before we knew all that we know now, we already recognized that buds that had a heavier blanket of sticky, shiny crystals typically led to a tastier and stonier experience.

Taking a Closer Look at Trichomes

Once we started growing our own cannabis we began to recognize those crystals for what they actually were — trichomes, the microscopic mushroom-shaped outgrowths that produce and house the very cannabinoid and terpene profiles that differentiate our favorite cultivars from one another.

There are quite literally hundreds of known cannabinoids, flavonoids, and terpenes which when combined in varying ratios produce the gassiest OGs, the heaviest purps, and the headiest hazes that we all know and love. The trichome is essentially the factory where all of those compounds are created and combined. Everything from the strength, the aroma, the flavor, and the overall effects of a particular strain of cannabis all emanate from the trichome.

The cannabis plant produces trichomes naturally on its flowers, fan leaves, branches and stalks. This coating of trichs is more abundant in the later stages of the plant’s growth cycle and is fueled by UV light as its contents continue to mature. This is the main reason why plants grown indoors typically have a higher THC content than the same strain grown outside. The consistent light source may boost the percentages, but we have learned that more trichomes do not always necessarily mean a “better” bud. Outdoor-grown weed lovers will contend that a broader spectrum of light (sunlight) leads to a broader spectrum of cannabinoids and terpenes.

Quality over Quantity

Trichome

PHOTO | Kenneth Kearney

Zooming in on the cannabis plant, you’ll notice that trichomes generally form in three different fashions, and that form tells us quite a bit about the contents within.

Capitate Stalked Trichomes

The average human eye can detect items as small as 40 micrometers, or microns. As the largest (50-100 microns wide) and most abundant of the three categories of trichs, the Capitate Stalked Trichome is visible to the naked eye and is what puts the bling in bag appeal. When the plant is being grown properly, these multicellular trichs will feature a strong stalk leading to a waxy, round head. It is in that top floor that top-shelf trichs are formed.

Capitate Sessile Trichomes

Slightly less abundant in number than their Stalked counterparts, the Capitate Sessile Trichome will resemble a stunted version of them. They still play an important role in the ‘entourage effect’ that we get when we consume the full spectrum of cannabinoids.

Bulbous Trichomes

At about a quarter of the width of a human hair, you are 100% more likely to notice a human hair in your bag of weed than you will the Bulbous Trichomes blanketing your buds. Even without a stalk, these basic microscopic bulbs also contribute to the full spectrum that many medicinal users seek. Like all trichomes, the abundance of these sticky outgrowths also serves to deter pests, particularly on plants grown outdoors and in greenhouses.

Harvesting Success

As a grower, it is crucial to become familiar with the ideal growth cycle of the strains in your stable. The difference between chopping plants down on Day 58 or on Day 64 can change an uplifting Sativa-like hybrid into a couch-locking sleep aid and that has way more to do with the maturity of the trichomes than it does the cultivar or phenotype.

There is a fine line between maturing and degrading and most cultivators make use of a handheld microscopic lens, perhaps a 50x zoom, to monitor the development of their plants’ trichs as they get closer to their target harvest date.

Though this can vary from strain to strain, clear heads on trichs generally indicate that the plant Is not fully mature. THC and other desirable cannabinoids are being formed, but be patient.

Once the trichomes display a milky or cloudy composition under the scope the plant can be harvested and the resulting buds and concentrates should deliver as heady and uplifting of an effect as the phenotype allows.

Over the course of the next few days, the observant grower will notice swaths of trichs turning to an amber color. It is very common for growers to chop their crop as soon as they determine that 30% of their heads are amber. With many strains, this will deliver the blend of heady vs. heavy that most consumers are after but it definitely varies.

For example, Beard Bros. Pharms is known for our superior cut of Extreme Cream which is absolutely caked in glistening trichs. Bill Levers, co-founder, reveals some insider information on their phenomenal phenotype.

“We first began harvesting her at just over eight weeks when her trichomes first began turning amber from cloudy,” said Levers. “We found that by allowing her another seven to 10 days and not harvesting until half or better of the trichomes were turning amber, that we received a much more desirable terpene and cannabinoid profile.”

As a consumer, it is important to recognize that trichomes continue to degrade even after the buds are trimmed, cured, packaged and sold. Factors like ambient temperature, light, physical agitation, and even time will age your trichs. What is the best solution? Smoke them before that happens!

For real though, any grower worth their weight in worm castings will take great care in producing potent trichs and it’s up to us to appreciate them. Regardless of how big the cannabis industry eventually becomes, it all begins in the dome of the trichome.

Beard Bros. Pharms has earned their reputation as a trusted source for cannabis news, content creation, and culture preservation. With decades of experience in cultivation and marketing, their fearless voice for the plant includes advocacy for veterans, inmates, people of color, and anyone else who has been oppressed by generations of cannabis prohibition.

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